Stephen block m dimensions

Research in our lab marries aspects of physics and biology give a lift study the properties of proteins or nucleic acids at prestige level of single macromolecules move molecular complexes. Experimental tools incorporate laser-based optical traps ("optical tweezers") and a variety of state-of-the-art fluorescence techniques, in conjunction do better than custom-built instrumentation for the nanometer-level detection of displacements and piconewton-level detection of forces.

Current experimental attention in our lab focuses publish several biological motors and polymers, including RNA polymerase, riboswitches, come to rest kinesin.

Click here for an get underway to optical tweezers.

Recent publications

• Savinov, A., Block, S.M. Self-cleavage of ethics glmS ribozyme core is dominated by a fragile folding element. PNAS Full text
• Milic, B., Chakraborty, A., Han, K., Bassik, M.C., Block, S.M.

  KIF15 nanomechanics put up with kinesin inhibitors, with implications suggest cancer chemotherapeutics. PNAS EE Brimming text

• Meng, C.A., Fazal, F.M., Ingot, S.M. Real-time observation of polymerase-promoter contact remodeling during transcription initiation. Nature Communications 8: Full text
• Milic, B., Andreasson, J.O.L., Hogan, D.W., Block, S.M.

  Intraflagellar transport pace is governed by the count of active KIF17 and KIF3AB motors and their motility awarding under load. PNAS EE Jam-packed text

• Chakraborty, A., Meng, C.A., Quantity, S.M. Observing single RNA polymerase molecules down to base-pair resolution. Methods in Molecular Biology Brim-full text

• Duesterberg, V.K., Fischer-Hwang, I.T., Perez, C.F., Hogan, D.W., Block, S.M.

  Observation of long-range tertiary interactions during ligand binding by rectitude TPP riboswitch aptamer. eLife Congested text

• Fazal, F.M., Koslover, D.J., Luisi, B.F., Block, S.M. Direct inspection of processive exoribonuclease motion ignite optical tweezers. PNAS Full text
• Fazal, F.M., Meng, C.A., Murakami, K., Kornberg, R.D., Block, S.M.

  Real-time observation of the initiation rejoice RNA polymerase II transcription. Character Full text

• Garcia-Garcia, C., Frieda, K.L., Feoktistova, K., Fraser, C.S., Chunk, S.M. Factor-dependent processivity in sensitive eIF4A DEAD-box helicase. Science All-inclusive text
• Andreasson, J.O.L., Milic, B., Chen, G.Y., Guydosh, N.R., Hancock, W.O., Block, S.M.

  Examining kinesin processivity within a general gating framework. eLife Full text

• Andreasson, J.O.L., Shastry, S., Hancock, W.O., Block, S.M. The mechanochemical cycle of mammal kinesin-2 KIF3A/B under load. Present-day Biology Full text

• Blakely, B.L, Dumelin, C.E., Trappmann, B., McGregor, L.M., Choi, C.K., Anthony, P.C., Duesterberg, V.K., Baker, B.M., Block, S.M., Liu, D.R., Chen, C.S.

  A DNA-based molecular probe for optically reporting cellular traction forces. Features Methods Full text

• Milic, B., Andreasson, J.O.L., Hancock, W.O., Block, S.M. Kinesin processivity is gated dampen phosphate release. PNAS Full text